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The Microinjection of sperm into the egg (ICSI) aims to facilitate fertilization by overcoming obstacles that sperm encounter in their attempt to penetrate the egg and achieve fertilization. It involves the direct microinjection of a spermatozoon into the interior of the oocyte.
It is performed in the laboratory by the embryologist, who uses micromanipulators to select a spermatozoon, pierce the cell membrane of the oocyte, and introduce the spermatozoon into its interior.
This technique is reserved for cases of severe male infertility or previous failure in IVF (In Vitro Fertilization).
Procedure Steps:
1. Consultation
Assessment of the patient’s condition, diagnosis, and establishment of the treatment plan.
2. Stimulation
Administration of drugs to stimulate the ovaries and ultrasound monitoring 2/3 times to assess the number and size of follicles. When the follicles reach the desired size, the procedure is scheduled, and an injection of hCG is required to induce oocyte maturation, approximately 36 hours before.
3. Follicular Aspiration
The procedure, with a maximum duration of approximately 15 minutes, involves the collection of matured oocytes and is performed in the operating room under sedation to minimize discomfort for the patient.
4. Oocyte Selection
After the collection of oocytes, they are chosen based on their potential to generate an embryo.
5. Sperm Collection
Sperm is collected. Donor sperm may also be used in appropriate cases.
6.Sperm Selection
Spermatozoa are prepared and selected based on their mobility and morphology.
7. Intracytoplasmic Injection
Intracytoplasmic injection is performed, introducing the spermatozoon into the previously selected egg
8.Embryonic Culture
The resulting embryos, after fertilization of the oocytes, are assessed daily based on their morphology and division rate, considering both the quality and quantity of the embryos.
9.Embryonic Transfer
The successfully fertilized in vitro embryo is placed inside the uterus.
10.Pregnancy Test
Blood analysis (βhCG) is performed to confirm pregnancy.
11.Pregnancy
Effectiveness of the performed technique.
