in Vitro Fertilization

in Vitro Fertilization (IVF) is an Assisted Reproductive Technology technique, which consists of the union of the female and male gametes in the laboratory. The ova are placed together with the spermatozoa in suitable culture media and fertilization occurs spontaneously.

This technique was initially used in women with obstructed tubes (tubal factor), but is currently applied in many other cases (mild male factor, endometriosis, cervical factor and infertility without apparent cause).

Procedure stages:

Medical Appointment

Assessment of the patient condition and establishment for medical treatment.

In vitro fertilization

IVF consists of placing oocytes on culture plate together with selected spermatozoa.

Embryo Vitrification

Surplus embryos, that will not be used for the ongoing fertilization cycle, will be frozen by rapid freezing in liquid nitrogen.


Administration of drugs for ovarian stimulation and ultrasound control 2/3 times, to measure the number and size of the follicles.
When the follicles reach the desired size, the procedure is scheduled and an hCG injection is required for induction of oocyte maturation about 36 hours earlier.

Follicular puncture

The procedure, with a maximum duration of approximately 15 minutes, consists of the collection of the matured oocytes, being performed in an operating room, under sedation, in order to minimize discomfort for the patient.

Sperm Collection

Sperm collection is done.
Donor sperm can also be used in the indicated cases.

Spermatozoa Selection

Spermatozoa are prepared and selected taking into account mobility and morphology.

Embryo Culture

The resulting embryos, after fertilization of the oocytes, are evaluated daily taking into account their morphology and rhythm of division, ie quality and quantity of the embryos.

Embryo Transfer

The embryo fertilized in vitro is successfully placed inside the uterus.

Pregnancy test

Blood analysis (βhCG) is performed to confirm pregnancy.


Efficacy of the technique performed.